PCR-Single-Strand Conformational Polymorphism Method for Rapid Detection of Rifampin-Resistant Mycobacterium tuberculosis : Systematic Review and Meta-Analysis
نویسندگان
چکیده
منابع مشابه
PCR-single-strand conformational polymorphism method for rapid detection of rifampin-resistant Mycobacterium tuberculosis: systematic review and meta-analysis.
The reference standard methods for drug susceptibility testing of Mycobacterium tuberculosis, such as culture on Lowenstein-Jensen or Middlebrook 7H10/11 medium, are very slow to give results; and due to the emergence of multidrug-resistant M. tuberculosis and extensively drug-resistant M. tuberculosis, there is an urgent demand for new, rapid, and accurate drug susceptibility testing methods. ...
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Either PCR-mediated single strand conformation polymorphism (SSCP) analysis or DNA sequencing of rpoB DNA (157 bp) can be used as a rapid screening method for the detection of mutations related to the rifampin resistance of Mycobacterium tuberculosis. However, due to the nonspecific amplification of rpoB DNA from nontuberculous mycobacteria these methods cannot be directly applied to clinical s...
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OBJECTIVE To rapidly detect rifampin resistance in Mycobacterium tuberculosis isolates causing meningitis in northeast Iran. METHODS This study presents the results of a polymerase chain reaction-single strand conformation polymorphism (PCR-SSCP) analysis for the evaluation of rifampin resistance directly from the CSF of 47 patients strongly suspicious to have tuberculosis meningitis in Emam ...
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متن کاملAllele-specific rpoB PCR assays for detection of rifampin-resistant Mycobacterium tuberculosis in sputum smears.
We describe an allele-specific PCR assay to detect mutations in three codons of the rpoB gene (516, 526, and 531) in Mycobacterium tuberculosis strains; mutations in these codons are reported to account for majority of M. tuberculosis clinical isolates resistant to rifampin (RIF), a marker of multidrug-resistant tuberculosis (MDR-TB). Three different allele-specific PCRs are carried out either ...
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ژورنال
عنوان ژورنال: Journal of Clinical Microbiology
سال: 2010
ISSN: 0095-1137,1098-660X
DOI: 10.1128/jcm.00960-10